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BrdU antibody

Cat.#: 107023

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Product Information

  • Product Name
    BrdU antibody
  • Documents
  • Description
    BrdU Mouse Monoclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in mouse spleen tissue.
  • Tested applications
    ELISA, IF, IHC
  • Species reactivity
    Human, Mouse, Rat; other species not tested.
  • Alternative names
    5-bromo-2-deoxyuridine antibody; BrDu antibody; BrdUrd antibody; Bromodeoxyuridine antibody; BUdR antibody
  • Isotype
    Mouse IgG2a
  • Preparation
    This antibody was obtained by immunization of Compound. Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    IHC: 1:200-1:1000

    IF: 1:100-1:500

  • Validations

    Immunohistochemistry of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using Catalog No:107023(BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded mouse spleen tissue slide (with/without BrdU feeding) using Catalog No:107023(BrDu Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using Catalog No:107023(BrdU Antibody) at dilution of 1:300 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using Catalog No:107023(BrdU Antibody) at dilution of 1:300 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using Catalog No:107023(BrdU Antibody) at dilution of 1:300 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

    Immunofluorescent analysis of HeLa cells (treated with 0.03 mg/ml BrdU for 2 hours) using Catalog No:107023(BrdU Antibody) at dilution of 1:300 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)

  • Background
    Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.
  • References
    • He Y, Cao X, Liu X. Quercetin reverses experimental pulmonary arterial hypertension by modulating the TrkA pathway. Experimental cell research. 339(1):122-34. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"