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Product Name
BRD8 antibody
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Description
BRD8 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in HeLa cells, human brain tissue. Observed molecular weight by Western-blot: 120 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human; other species not tested.
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Alternative names
BRD8 antibody; bromodomain containing 8 antibody; p120 antibody; SMAP antibody; SMAP2 antibody; TrCP120 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BRD8 recombinant protein (Accession Number: BC008039). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:1000
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117230(BRD8 antibody) at dilution of 1:500
IP Result of anti-BRD8 (IP:Catalog No:117230, 4ug; Detection:Catalog No:117230 1:300) with HeLa cells lysate 1200ug.
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Background
BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].
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