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Product Name
BRD7 antibody
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Description
BRD7 Rabbit Polyclonal antibody. Positive WB detected in HeLa cells, L02 cells, mouse cerebellum tissue, mouse lung tissue, rat brain tissue, SH-SY5Y cells. Positive IHC detected in human colon cancer tissue, human cervical cancer tissue. Observed molecular weight by Western-blot: 70-74kd
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
75 kDa bromodomain protein antibody; BP75 antibody; BRD7 antibody; bromodomain containing 7 antibody; CELTIX1 antibody; NAG4 antibody; Protein CELTIX 1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BRD7 recombinant protein (Accession Number: NM_013263). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117229(BRD7 antibody) at dilution of 1:500
Immunohistochemistry of paraffin-embedded human cervical cancer tissue slide using Catalog No:117229(BRD7 Antibody) at dilution of 1:50 (under 40x lens)
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Background
BRD7 (bromodomain containing 7), is a subunit of poly-bromo-associated BRG1-associated factor (PBAF)-specific Swi/Snf chromatin remodeling complexes.BRD7 is proposed as a tumor suppressor protein, since its identification as an important component of both functional p53 and BRCA1 (breast cancer 1, early onset) pathways. BRD7 could down-regulate the GSK3beta phosphotransferase activity as dephosphorylation of GSK3B at 'Tyr-216', thus activate the Wnt signaling pathway in a DVL1-dependent manner.
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References
- Kaeser MD, Aslanian A, Dong MQ, Yates JR, Emerson BM. BRD7, a novel PBAF-specific SWI/SNF subunit, is required for target gene activation and repression in embryonic stem cells. The Journal of biological chemistry. 283(47):32254-63. 2008.
- Harte MT, O'Brien GJ, Ryan NM. BRD7, a subunit of SWI/SNF complexes, binds directly to BRCA1 and regulates BRCA1-dependent transcription. Cancer research. 70(6):2538-47. 2010.
- Liu H, Li X, Niu Z. Preparation of polyclonal antibody specific for BRD7 and detection of its expression pattern in the human fetus. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 56(6):531-8. 2008.
- Xu Y, Cao W, Zhou M. Inactivation of BRD7 results in impaired cognitive behavior and reduced synaptic plasticity of the medial prefrontal cortex. Behavioural brain research. 286:1-10. 2015.
- Zhang Q, Wei L, Yang H. Bromodomain containing protein represses the Ras/Raf/MEK/ERK pathway to attenuate human hepatoma cell proliferation during HCV infection. Cancer letters. 371(1):107-16. 2016.
- Wang H, Zhao R, Guo C. Knockout of BRD7 results in impaired spermatogenesis and male infertility. Scientific reports. 6:21776. 2016.
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