-
Product Name
BRD3 Polyclonal Antibody
- Documents
-
Description
Polyclonal antibody to BRD3
-
Tested applications
WB, IHC, IF
-
Species reactivity
Human, Rat
-
Alternative names
BRD3 antibody; ORFX antibody; RING3L antibody; bromodomain containing 3 antibody
-
Isotype
Rabbit IgG
-
Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 150-305 of human BRD3 (NP_031397.1).
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
-
Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
-
Applications
WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - BRD3 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BRD3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 5s.
Immunohistochemistry - BRD3 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat testis using BRD3 antibody at dilution of 1:100 (40x lens).
-
Background
Chromatin reader that recognizes and binds hyperacetylated chromatin and plays a role in the regulation of transcription, probably by chromatin remodeling and interaction with transcription factors. Regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"