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Product Name
BRD3 antibody
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Description
BRD3 Rabbit Polyclonal antibody. Positive IHC detected in human ovary tumor tissue, human gliomas tissue. Positive IP detected in HeLa cells. Positive WB detected in human brain tissue, COLO 320 cells, HeLa cells. Observed molecular weight by Western-blot: 95-105 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BRD3 antibody; bromodomain containing 3 antibody; FLJ23227 antibody; FLJ41328 antibody; KIAA0043 antibody; ORFX antibody; RING3 like protein antibody; RING3L antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BRD3 recombinant protein (Accession Number: BC032124). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:117228(BRD3 antibody) at dilution of 1:200
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:117228(BRD3 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary tumor using Catalog No:117228(BRD3 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-BRD3 (IP:Catalog No:117228, 4ug; Detection:Catalog No:117228 1:500) with HeLa cells lysate 4000ug.
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Background
Brd3 belong to bromodomain protein (BET)family, which recognizes and binds to lysines of histones, and assiciation with acetylated nucleosomes. It facilitates the passage of pol II through nucleosomal templates in a histone acetylation-dependent manner
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References
- Zhang L, Deng T, Li X. microRNA-141 is involved in a nasopharyngeal carcinoma-related genes network. Carcinogenesis. 31(4):559-66. 2010.
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