BRAP Monoclonal Antibody

Cat.#: 166232

Special Price 232.3 USD

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Product Information

  • Product Name
    BRAP Monoclonal Antibody
  • Documents
  • Description
    Monoclonal antibody to BRAP
  • Tested applications
    WB
  • Species reactivity
    Human
  • Alternative names
    BRAP antibody; BRAP2 antibody; IMP antibody; RNF52 antibody; BRCA1-associated protein antibody
  • Isotype
    Mouse IgG
  • Preparation
    Antigen: Recombinant protein of human BRAP
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - BRAP Monoclonal Antibody

    Western blot - BRAP Monoclonal Antibody

    Western blot analysis of extracts of various cell lines, using BRAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.

  • Background
    Negatively regulates MAP kinase activation by limiting the formation of Raf/MEK complexes probably by inactivation of the KSR1 scaffold protein. Also acts as a Ras responsive E3 ubiquitin ligase that, on activation of Ras, is modified by auto-polyubiquitination resulting in the release of inhibition of Raf/MEK complex formation. May also act as a cytoplasmic retention protein with a role in regulating nuclear transport.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"