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Product Name
BPIL1 antibody
- Documents
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Description
BPIL1 Rabbit Polyclonal antibody. Positive IF detected in A549 cells. Positive IHC detected in human lung cancer tissue, human tonsillitis tissue. Positive WB detected in A549 cells. Observed molecular weight by Western-blot: 49kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human; other species not tested.
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Alternative names
BPIL1 antibody; C20orf184 antibody; dJ726C3.2 antibody; LPLUNC2 antibody; RYSR antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BPIL1 recombinant protein (Accession Number: NM_025227). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:117218(BPIL1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:117218(BPIL1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung cancer using Catalog No:117218(BPIL1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of A549 cells using Catalog No:117218(BPIL1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Bactericidal/permeability-increasing protein-like 1 (BPIL1, also known as LPLUNC2 or BPIFB2) is a member of the lipid transfer (LT)/lipopolysaccharide binding protein (LBP) family. LT/LBP proteins are structurally related proteins capable of binding phospholipids and lipopolysaccharides (PMID: 12185532). The gene of BPIL1 maps to Chromosome 20q11, and encodes a 458-amino acid protein with a calculated molecular weight of 49 kDa. It is highly expressed in hypertrophic tonsils. BPIL1 has been identified as a nasal mucous protein which may be involved in immune response in the nose against microbial infections (PMID: 15996010). Reduced expression of antimicrobial PLUNC proteins (LPLUNC1 and LPLUNC2) has been reported in nasal polyp tissues of patients with chronic rhinosinusitis (PMID: 22676062).
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References
- Seshadri S, Lin DC, Rosati M. Reduced expression of antimicrobial PLUNC proteins in nasal polyp tissues of patients with chronic rhinosinusitis. Allergy. 67(7):920-8. 2012.
- Foster MW, Thompson JW, Que LG. Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure. Journal of proteome research. 12(5):2194-205. 2013.
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