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Product Name
BOLA1 antibody
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Description
BOLA1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human lung tissue, human heart tissue, human kidney tissue, human placenta tissue, human spleen tissue. Positive IP detected in HepG2 cells. Positive WB detected in HepG2 cells, HEK-293 cells. Observed molecular weight by Western-blot: 14 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
bolA homolog 1 (E. coli) antibody; BolA like protein 1 antibody; BOLA1 antibody; CGI 143 antibody; hBolA antibody; RP11 196G18.18 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BOLA1 recombinant protein (Accession Number: NM_016074). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:117211(BOLA1 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human lung using Catalog No:117211(BOLA1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human lung using Catalog No:117211(BOLA1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-BOLA1 (IP:Catalog No:117211, 4ug; Detection:Catalog No:117211 1:800) with HepG2 cells lysate 3480ug.
Immunofluorescent analysis of HepG2 cells using Catalog No:117211(BOLA1 Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
BOLA1 represents bolA homolog 1 (E. coli)
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