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Product Name
BNIP3L Polyclonal Antibody
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Description
Polyclonal antibody to BNIP3L
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
BNIP3L antibody; BNIP3a antibody; NIX antibody; BCL2 interacting protein 3 like antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human BNIP3L (NP_004322.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - BNIP3L Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BNIP3L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 30s.
Immunohistochemistry - BNIP3L Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using BNIP3L antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BNIP3L Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat brain using BNIP3L antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BNIP3L Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human lung cancer using BNIP3L antibody at dilution of 1:100 (40x lens).
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Background
Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates in mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. The physical interaction of SPATA18/MIEAP, BNIP3 and BNIP3L/NIX at the mitochondrial outer membrane regulates the opening of a pore in the mitochondrial double membrane in order to mediate the translocation of lysosomal proteins from the cytoplasm to the mitochondrial matrix. May function as a tumor suppressor.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"