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BNIP1 antibody

Cat.#: 108473

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Special Price 239.0 USD

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Product Information

  • Product Name
    BNIP1 antibody
  • Documents
  • Description
    BNIP1 Rabbit Polyclonal antibody. Positive IP detected in mouse brain tissue. Positive WB detected in human brain tissue, HeLa cells, human liver tissue, mouse skeletal muscle tissue, rat brain tissue. Observed molecular weight by Western-blot: 26 kDa
  • Tested applications
    ELISA, WB, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    BNIP1 antibody; NIP1 antibody; SEC20 antibody; SEC20L antibody; TRG 8 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of BNIP1 recombinant protein (Accession Number: NM_001205). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:117208(BNIP1 antibody) at dilution of 1:500

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:117208(BNIP1 antibody) at dilution of 1:500

    IP Result of anti-BNIP1 (IP:Catalog No:117208, 3ug; Detection:Catalog No:117208 1:500) with mouse brain tissue lysate 6000ug.

    IP Result of anti-BNIP1 (IP:Catalog No:117208, 3ug; Detection:Catalog No:117208 1:500) with mouse brain tissue lysate 6000ug.

  • Background
    BNIP1 is a member of the BCL2/adenovirus E1B 19 kDa-interacting protein (BNIP) family. The encoded protein is predominantly localized to the endoplasmic reticulum (ER), is a pro-apoptotic Bcl-2 homology domain 3 (BH3)-only protein. BNIP1, also called SEC20L, is a component of a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L and SEC22B which is involved in apoptosis and ER membrane fusion. Recent reports showed that expression of BNIP1 induced mitochondrial fragmentation in a BH3 domain-dependent manner via increasing dynamin-related protein 1 (Drp1) expression. RNF185 is a mitochondrial ubiquitin E3 ligase that regulates selective mitochondrial autophagy, BNIP1 colocalizes with RNF185 at mitochondria and is polyubiquitinated by RNF185 through K63-based ubiquitin linkage in vivo and modulatemitochondrial homeostasis through autophagy.
  • References
    • Tang F, Wang B, Li N. RNF185, a novel mitochondrial ubiquitin E3 ligase, regulates autophagy through interaction with BNIP1. PloS one. 6(9):e24367. 2011.
    • Wang P, Wu Y, Li Y, Zheng J, Tang J. A novel RING finger E3 ligase RNF186 regulate ER stress-mediated apoptosis through interaction with BNip1. Cellular signalling. 25(11):2320-33. 2013.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"