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Product Name
BMSC UbP antibody
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Description
BMSC UbP Rabbit Polyclonal antibody. Positive IHC detected in human gliomas tissue. Positive IF detected in HepG2 cells. Positive IP detected in HeLa cells. Positive WB detected in HepG2 cells, HeLa cells, Jurkat cells, mouse testis tissue, rat testis tissue. Observed molecular weight by Western-blot: 41 kDa
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Tested applications
ELISA, WB, IHC, IP, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BMSC UbP antibody; BMSCUBP antibody; TCBA1 antibody; Ubiquitin like protein 7 antibody; Ubiquitin like protein SB132 antibody; UBL7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BMSC UbP recombinant protein (Accession Number: NM_032907). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:117205(UBL7 antibody) at dilution of 1:400
IP Result of anti-UBL7 (IP:Catalog No:117205, 4ug; Detection:Catalog No:117205 1:1000) with HeLa cells lysate 2000ug.
Immunohistochemical of paraffin-embedded human gliomas using Catalog No:117205(UBL7 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells using Catalog No:117205(UBL7 Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Ubiquitin is an important cellular signal that targets proteins for degradation or regulates their functions. UBL7 (ORF name SB132) encodes ubiquitin-like protein7 also named bone marrow stromal cell ubiquitin-like protein (BMSC-UbP) capable of binding ubiquitin. BMSC-UbP protein is derived from bone marrow stromal cells containing a ubiquitin-associated (UBA) domain at the C terminus that has been implicated in linking cellular processes and the ubiquitin system. BMSC-UbP mRNA is widely expressed in human multiple tissues and various tumor cell lines and is decreased in BMSC stimulated with phorbol myristate acetate (PMA) suggesting a role in regulation of BMSC function.
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