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Product Name
BMI1 antibody
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Description
BMI1 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Positive IP detected in U-937 cells. Positive WB detected in U-937 cells, K-562 cells, mouse brain tissue, NCCIT cell. Observed molecular weight by Western-blot: 43-45 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BMI1 antibody; PCGF4 antibody; Polycomb complex protein BMI 1 antibody; RING finger protein 51 antibody; RNF51 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BMI1 recombinant protein (Accession Number: NM_005180). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:117193(BMI1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:117193(BMI1 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of HepG2 cells, using Catalog No:117193 and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
IP Result of anti-BMI1 (IP:Catalog No:117193, 4ug; Detection:Catalog No:117193 1:500) with U-937 cells lysate 4000ug.
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Background
BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells The antibody 10832-1-AP detected proteins of 45-48kda, which may include phosphorylated isoforms of the protein.
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References
- Wu Z, Min L, Chen D. Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma. PloS one. 6(2):e14648. 2011.
- Wang HB, Liu GH, Zhang H. Sp1 and c-Myc regulate transcription of BMI1 in nasopharyngeal carcinoma. The FEBS journal. 280(12):2929-44. 2013.
- Nishida Y, Maeda A, Chachad D. Preclinical activity of the novel B-cell-specific Moloney murine leukemia virus integration site 1 inhibitor PTC-209 in acute myeloid leukemia: Implications for leukemia therapy. Cancer science. 106(12):1705-13. 2015.
- Kim MO, Chawla P, Overland RP, Xia E, Sadri-Vakili G, Cha JH. Altered histone monoubiquitylation mediated by mutant huntingtin induces transcriptional dysregulation. The Journal of neuroscience : the official journal of the Society for Neuroscience. 28(15):3947-57. 2008.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"