Product Name

BMI1 antibody

Documents

Description

BMI1 Mouse Monoclonal antibody. Positive WB detected in U-937 cells. Positive IP detected in HEK-293 cells. Positive IHC detected in human lymphoma tissue. Positive IF detected in COS-7 cells. Observed molecular weight by Western-blot: 37 kDa

Tested applications

ELISA, WB, IHC, IF, IP

Species reactivity

Human, Mouse; other species not tested.

Alternative names

BMI1 antibody; PCGF4 antibody; Polycomb complex protein BMI 1 antibody; RING finger protein 51 antibody; RNF51 antibody

Isotype

Mouse IgG2b

Preparation

This antibody was obtained by immunization of BMI1 recombinant protein (Accession Number: NM_005180). Purification method: Protein A purified.

Clonality

Monoclonal

Formulation

PBS with 0.02% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

U-937 cells were subjected to SDS PAGE followed by western blot with Catalog No:107019(BMI1 Antibody) at dilution of 1:1000

IP Result of anti-BMI1 (IP:Catalog No:107019, 3ug; Detection:Catalog No:107019 1:1000) with HEK-293 cells lysate 1800ug.

Immunohistochemistry of paraffin-embedded human lymphoma tissue slide using Catalog No:107019(BMI1 Antibody) at dilution of 1:50 under 40x lens

Immunofluorescent analysis of COS-7 cells using Catalog No:107019(BMI1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Mouse IgG

Background

BMI- 1 is one of polycomb group genes, which together with Ring1 strongly enhances the E3 ubiquitin ligase activity of the Ring2 catalytic subunit. Bmi1 plays an important role in the regulation of cell proliferation and senescence through repression of the p16Ink4a and p19Arf genes and is required for maintenance of adult hematopoietic and neural stem cells. The antibody reacts with the 37kd BMI1 protein.

BMI1 antibody

Product Information

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