BMAP-27 peptide
Not For Human Use, Lab Use Only.
Cat.#: 318783
Optional Service:
TFA RemovalWhat's this?
Special Price 225.2 USD
Availability:
3 weeks
-
Product Name
BMAP-27 peptide
- Documents
-
Quantity/Unit
1 Vial
-
Sequence
H-GRFKRFRKKFKKLFKKLSPVIPLLHL-Amidation
-
Three letter code
H-Gly-Arg-Phe-Lys-Arg-Phe-Arg-Lys-Lys-Phe-Lys-Lys-Leu-Phe-Lys-Lys-Leu-Ser-Pro-Val-Ile-Pro-Leu-Leu-His-Leu-Amidation
-
Length (aa)
26
-
Peptide Purity (HPLC)
95.2
-
Molecular Formula
C158H263N45O27
-
Molecular Weight
3225.05
-
Source
Synthetic
-
Additional Information
BMAP-27 is a membranolytic helical peptide. Upon interaction with bacterial membranes, BMAP-27 acquires an amphipathic α-helical conformation, with the polar sector of the cylinder characterized by the presence of numerous positively charged residues, and the opposite one having a markedly hydrophobic character. This configuration favours interaction with, and insertion into, the bacterial membrane, and the peptides then aggregate to form pores and/or disaggregates the membranes by the so-called “carpet mechanism” in a concentration dependent manner. BMAP-27 is induced to adopt a α-helical conformation in the presence of EPOLs from BF produced by all three strains, KpTs101, KpTs113, and KpMn7, but to different extents.
-
Storage Guidelines
Normally, this peptide will be delivered in lyophilized form and should be stored in a freezer at or below -20 °C. For more details, please refer to the manual:Handling and Storage of Synthetic Peptides
-
References
- Bellich B, Lagatolla C, Tossi A, Benincasa M, Cescutti P, Rizzo R. Influence of Bacterial Biofilm Polysaccharide Structure on Interactions with Antimicrobial Peptides: A Study on Klebsiella pneumoniae. Int J Mol Sci. 2018;19(6)
-
About TFA salt
Trifluoroacetic acid (TFA) is a strong acid, which is commonly used to cleave synthesized peptides from solid-phase resins and is also used to improve HPLC performance in the peptide purification step. By default, custom peptides are delivered as lyophilized TFA salts, and can contain as much as 10-45% TFA.
TFA in custom peptides can cause inexplicable discrepancies in subsequent assay data. For instance, TFA in nM concentrations has been shown to interfere with cellular assays, inhibiting cellular proliferation in some instances, and increasing cell viability in others . It has also been found to be an unintended allosteric modulator of the glycine receptor, GlyR.
TFA Removal Service is recommended for:
- Peptides that will be used in cellular assays
- Peptides that will be used as APIs or in manufactured products
- For hydrophilic peptides containing numerous basic residues
Peptide Property
- Analysed Sequence:H-GRFKRFRKKFKKLFKKLSPVIPLLHL-Amidation
- Chemical Formula:C158H263N45O27
- Sequence length:26
- Extinction coefficient:0 M-1cm-1
- GRAVY:-0.37
- Mw average:3225.05
- Theoretical pI:12.73
- Data Source:Peptide Property Calculator
GRAVY = grand average of hydropathy
X: Hydrophobic uncharged residues, like F I L M V W A and P
X: Basic residues, like R K H
X: Acidic residues, like D E
X: Polar uncharged residues, like G S T C N Q and Y
Related Products / Services
• Peptide Services: NovoPro's peptide synthesis services include standard chemical peptide synthesis, peptide modification, peptide libraries, and recombinant peptide expression.
• Standard Peptide Synthesis: NovoPro offers quality peptides at the most competitive prices in the industry, starting at $3.20 per amino acid. NovoPro provides PepBox – Automatic Quote Tool for online price calculation.
• Peptide Modifications: NovoPro offers a wide range of peptide modification services including isotope labeling (2H, 15N, and 13C), multiple disulfide bonds, multiple phosphorylations, KLH, BSA, ovalbumin, amidation, acetylation, biotin, FITC, etc.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"