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Product Name
BLZF1 Polyclonal Antibody
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Description
Polyclonal antibody to BLZF1
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
BLZF1 antibody; GOLGIN-45 antibody; JEM-1 antibody; JEM-1s antibody; JEM1 antibody; golgin-45 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-400 of human BLZF1 (NP_003657.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - BLZF1 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BLZF1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - BLZF1 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using BLZF1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - BLZF1 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using BLZF1 antibody . Blue: DAPI for nuclear staining.
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Background
Required for normal Golgi structure and for protein transport from the endoplasmic reticulum (ER) through the Golgi apparatus to the cell surface.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"