Product Name

BID antibody

Documents

Description

BID Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human colon cancer tissue, human pancreas tissue. Positive WB detected in HepG2 cells, A431 cells, A549 cells, HEK-293 cells, HeLa cells, human brain tissue, Jurkat cells, Tunicamycin treated HeLa cells. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 22 kDa

Tested applications

ELISA, WB, IHC, IP, IF

Species reactivity

Human; other species not tested.

Alternative names

BID antibody; FP497 antibody; p11 BID antibody; p22 BID antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of BID recombinant protein (Accession Number: NM_001244567). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:500-1:5000

IP: 1:500-1:5000

IHC: 1:20-1:200

IF: 1:20-1:200

Validations

HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:117142(BID antibody) at dilution of 1:1000

IP Result of anti-BID (IP:Catalog No:117142, 3ug; Detection:Catalog No:117142 1:1000) with HeLa cells lysate 3000ug.

Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:117142(BID antibody) at dilution of 1:100 (under 10x lens)

Tunicamycin treated HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:117142(BID Antibody) at dilution of 1:1000

Immunofluorescent analysis of HeLa cells using Catalog No:117142(BID Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Background

BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)

References

Liu C, Gong K, Mao X, Li W. Tetrandrine induces apoptosis by activating reactive oxygen species and repressing Akt activity in human hepatocellular carcinoma. International journal of cancer. 129(6):1519-31. 2011.
Chen W, Hou J, Yin Y. alpha-Bisabolol induces dose- and time-dependent apoptosis in HepG2 cells via a Fas- and mitochondrial-related pathway, involves p53 and NFkappaB. Biochemical pharmacology. 80(2):247-54. 2010.
Gong K, Li W. Shikonin, a Chinese plant-derived naphthoquinone, induces apoptosis in hepatocellular carcinoma cells through reactive oxygen species: A potential new treatment for hepatocellular carcinoma. Free radical biology & medicine. 51(12):2259-71. 2011.
Hu M, Xu L, Yin L. Cytotoxicity of dioscin in human gastric carcinoma cells through death receptor and mitochondrial pathways. Journal of applied toxicology : JAT. 33(8):712-22. 2013.
Gong K, Xie J, Yi H, Li W. CS055 (Chidamide/HBI-8000), a novel histone deacetylase inhibitor, induces G1 arrest, ROS-dependent apoptosis and differentiation in human leukaemia cells. The Biochemical journal. 443(3):735-46. 2012.
Lei JC, Yu JQ, Yin Y, Liu YW, Zou GL. Alantolactone induces activation of apoptosis in human hepatoma cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 50(9):3313-9. 2012.
Jia Y, Ji L, Zhang S. Total flavonoids from Rosa Laevigata Michx fruit attenuates hydrogen peroxide induced injury in human umbilical vein endothelial cells. Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 50(9):3133-41. 2012.
Yu G, Jiang L, Xu Y. Silencing prion protein in MDA-MB-435 breast cancer cells leads to pleiotropic cellular responses to cytotoxic stimuli. PloS one. 7(11):e48146. 2012.

BID antibody

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