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Product Name
BID antibody
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Description
BID Mouse Monoclonal antibody. Positive WB detected in Jurkat cells, HeLa cells. Positive IF detected in HeLa cells. Observed molecular weight by Western-blot: 22 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
BID antibody; FP497 antibody; p11 BID antibody; p22 BID antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was obtained by immunization of BID recombinant protein (Accession Number: NM_001244567). Purification method: Protein G purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107018(BID Antibody) at dilution of 1:1000
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:107018(BID Antibody) at dilution of 1:1000
Immunofluorescent analysis of HeLa cells using Catalog No:107018(BID Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
BID, also named as p22 BID, can be cleaved into p11 BID, p13 BID and p15 BID. It is pro-apoptotic molecules. The major proteolytic product p15 BID allows the release of cytochrome c. BID-L, BID-EL and BID-ES induce ICE-like proteases and apoptosis. BID-S does not induce apoptosis. BID counters the protective effect of Bcl-2. (PMID:14583606)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"