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Product Name
beta Tubulin antibody
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Description
beta Tubulin Mouse Monoclonal antibody. Positive WB detected in RAW 264.7 cells. Positive IP detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human brain tissue, human breast cancer tissue, rat brain tissue. Observed molecular weight by Western-blot: 50-55 kDa
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human, Mouse, Rat; other species not tested.
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Alternative names
beta tubulin antibody; MC1R antibody; TUBB antibody; Tubulin-beta antibody
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Isotype
Mouse IgG2a
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Preparation
This antibody was obtained by immunization of beta Tubulin recombinant protein (Accession Number: NM_006086). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:2000-1:20000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
RAW 264.7 cells were subjected to SDS PAGE followed by western blot with Catalog No:117308 (Tubulin-beta Antibody) at different dilutions.
Immunofluorescent analysis of HepG2 cells using Catalog No:117308(Tubulin-beta Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:117308(Tubulin-beta Antibody) at dilution of 1:400 (under 10x lens)
Immunohistochemistry of paraffin-embedded human brain tissue slide using Catalog No:117308(Tubulin-beta Antibody) at dilution of 1:400 (under 40x lens)
IP Result of anti-Tubulin-beta (IP:Catalog No:117308, 5ug; Detection:Catalog No:117308 1:1000) with HeLa cells lysate 880ug.
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Background
There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart, once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha and beta tubulins have been widely used as loading controls. Tubulin expression may vary according to resistance to antimicrobial and antimitotic drugs.
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