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Product Name
beta Actin antibody
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Description
beta Actin Mouse Monoclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in multi-cells, A549 cells, CHO cells, HeLa. Positive FC detected in HeLa cells. Positive IF detected in HepG2 cells. Positive IHC detected in human kidney tissue, human brain tissue, human colon cancer tissue, human heart tissue. Observed molecular weight by Western-blot: 42 kDa
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Tested applications
ELISA, IHC, IF, WB, FC, IP
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Species reactivity
Human, Mouse, Rat, Hamster, Zebrafish; other species not tested.
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Alternative names
ACTB antibody; Actin antibody; actin antibody; beta antibody; Actin antibody; cytoplasmic 1 antibody; B actin antibody; Beta actin antibody; F actin antibody; PS1TP5BP1 antibody; β actin antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of recombinant protein (Accession Number: NM_001101). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:5000-1:50000
IP: 1:5000-1:50000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Western blot analysis of beta-actin in various tissues and cell lines using Proteintech antibody Catalog No:117302 at a dilution of 1:20000. (Exposure time: 10 seconds)
A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with Catalog No:117302 (Mouse anti Pan-actin antibody) at dilution of 1:10000.
WB result of anti-beta Actin monoclonal antibody (Catalog No:117302) with HeLa lysate at various dilutions.
IP Result of anti-beta actin (IP:Catalog No:117302, 3ug; Detection:Catalog No:117302 1:10000) with HeLa cells lysate 3100ug.
Immunofluorescent analysis of HepG2 cells, using beta actin antibody Catalog No: at 1:200 dilution and Rhodamine-labeled goat anti-mouse IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Immunohistochemical of paraffin-embedded human kidney using Catalog No:117302 at dilution of 1:500 (under 40x lens)
1X10^6 HeLa cells were stained with 0.2ug beta actin antibody (Catalog No:117302, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.
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Background
Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process.This antibody can recognize all the actins. The isotype of this antibody is IgG2b.
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References
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- Chen X, Shi X, Zhang X. Scutellarin attenuates hypertension-induced expression of brain Toll-like receptor 4/nuclear factor kappa B. Mediators of inflammation. 2013:432623. 2013.
- Chen YF, Dong Z, Xia Y. Nucleoside analog inhibits microRNA-214 through targeting heat-shock factor 1 in human epithelial ovarian cancer. Cancer science. 104(12):1683-9. 2013.
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- Zeng L, Wang W, Rong XF. Chondroprotective effects and multi-target mechanisms of Icariin in IL-1 beta-induced human SW 1353 chondrosarcoma cells and a rat osteoarthritis model. International immunopharmacology. 18(1):175-81. 2014.
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