-
Product Name
BDH1 antibody
- Documents
-
Description
BDH1 Rabbit Polyclonal antibody. Positive IP detected in mouse liver tissue. Positive WB detected in human liver tissue, human heart tissue, mouse liver tissue, rat brain tissue. Positive IHC detected in human liver tissue. Observed molecular weight by Western-blot: 31 kDa
-
Tested applications
ELISA, WB, IHC, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
BDH antibody; BDH1 antibody; SDR9C1 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of BDH1 recombinant protein (Accession Number: XM_017007007). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:1000
IHC: 1:20-1:200
-
Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:117112(BDH1 antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human liver using Catalog No:117112(BDH1 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:117112(BDH1 antibody) at dilution of 1:50 (under 40x lens)
IP Result of anti-BDH1 (IP:Catalog No:117112, 4ug; Detection:Catalog No:117112 1:300) with mouse liver tissue lysate 4000ug.
-
Background
BDH1 (3-hydroxybutyrate dehydrogenase, type 1) is a 343 amino acid protein that localizes to the mitochondrial matrix and belongs to the short-chain dehydrogenases/reductases (SDR) family. The deduced 343 amino acid protein contains a 46 residue leader peptide, which is cleaved to produce the mature form. It is a lipid-requiring mitochondrial enzyme with a specific requirement of phosphatidylcholine (PC).
-
References
- Cotter DG, Ercal B, d'Avignon DA, Dietzen DJ, Crawford PA. Impact of peripheral ketolytic deficiency on hepatic ketogenesis and gluconeogenesis during the transition to birth. The Journal of biological chemistry. 288(27):19739-49. 2013.
- Cotter DG, Ercal B, d'Avignon DA, Dietzen DJ, Crawford PA. Impairments of hepatic gluconeogenesis and ketogenesis in PPARα-deficient neonatal mice. American journal of physiology. Endocrinology and metabolism. 307(2):E176-85. 2014.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"