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Product Name
BCR antibody
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Description
BCR Rabbit Polyclonal antibody. Positive IHC detected in human skin tissue. Positive IF detected in HepG2 cells. Positive FC detected in HepG2 cells. Positive IP detected in HEK-293 cells. Positive WB detected in Y79 cells, COS-7 cells, HEK-293 cells, HeLa cells, K-562 cells. Observed molecular weight by Western-blot: 150-160 kDa
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Tested applications
ELISA, WB, IF, IHC, FC, IP
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Species reactivity
Human, Monkey; other species not tested.
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Alternative names
ALL antibody; BCR antibody; breakpoint cluster region antibody; CML antibody; D22S11 antibody; D22S662 antibody; FLJ16453 antibody; PHL antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BCR recombinant protein (Accession Number: BC066122). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
Y79 cells were subjected to SDS PAGE followed by western blot with Catalog No:117109(BCR antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human skin slide using Catalog No:117109(BCR Antibody) at dilution of 1:50
Immunohistochemistry of paraffin-embedded human skin slide using Catalog No:117109(BCR Antibody) at dilution of 1:50
Immunofluorescent analysis of HepG2 cells, using BCR antibody Catalog No:117109 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
IP Result of anti-BCR (IP:Catalog No:117109, 4ug; Detection:Catalog No:117109 1:1000) with HEK-293 cells lysate 3000ug.
1X10^6 HepG2 cells were stained with .2ug BCR antibody (Catalog No:117109, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The Bcr gene encodes for the break point cluster region protein(Bcr). Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. The protein has serine/threonine kinase activity and is a GTPase-activating protein for RAC1 and CDC42. A chromosomal aberration involving BCR has been found in patients with chronic myeloid leukemia.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"