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Product Name
BCO2 antibody
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Description
BCO2 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human liver cancer tissue. Positive WB detected in L02 cells, human liver tissue, mouse liver tissue. Observed molecular weight by Western-blot: 60-65kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
B DIOX II antibody; BCO2 antibody; Beta carotene dioxygenase 2 antibody; beta carotene oxygenase 2 antibody; FLJ34464 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BCO2 recombinant protein (Accession Number: NM_001256398). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:20-1:200
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Validations
L02 cells were subjected to SDS PAGE followed by western blot with Catalog No:117107(BCO2 antibody) at dilution of 1:800
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:117107(BCO2 antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:117107(BCO2 antibody) at dilution of 1:50 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using BCO2 antibody Catalog No:117107 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
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Background
Beta,beta-carotene 9',10'-oxygenase (BCO2) catalyzes the asymmetric oxidative cleavage of beta-carotene in carotene metabolism.The apocarotenals formed by this enzyme may be the precursors for the biosynthesis of retinoic acid or exert unknown physiological effects. BCDO2 plays an important role in protecting against carotenoid-induced mitochondrial dysfunction and has a broader substrate specificity than previously recognized(PMID:22147584). This antibody detects the N-terminal of BCO2.
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References
- Yu H, Wark L, Ji H. Dietary wolfberry upregulates carotenoid metabolic genes and enhances mitochondrial biogenesis in the retina of db/db diabetic mice. Molecular nutrition & food research. 57(7):1158-69. 2013.
- Lin D, He H, Ji H. Wolfberries potentiate mitophagy and enhance mitochondrial biogenesis leading to prevention of hepatic steatosis in obese mice: the role of AMP-activated protein kinase α2 subunit. Molecular nutrition & food research. 58(5):1005-15. 2014.
- Li B, Vachali PP, Gorusupudi A. Inactivity of human β,β-carotene-9',10'-dioxygenase (BCO2) underlies retinal accumulation of the human macular carotenoid pigment. Proceedings of the National Academy of Sciences of the United States of America. 111(28):10173-8. 2014.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"