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Product Name
BCMO1 antibody
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Description
BCMO1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive WB detected in COLO 320 cells, mouse colon tissue. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
BCMO1 antibody; Beta carotene dioxygenase 1 antibody; FLJ10730 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BCMO1 recombinant protein (Accession Number: NM_017429). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IHC: 1:20-1:200
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Validations
COLO 320 cells were subjected to SDS PAGE followed by western blot with Catalog No:117106(BCMO1 Antibody) at dilution of 1:300
Immunohistochemistry of paraffin-embedded human testis tissue slide using Catalog No:117106(BCMO1 Antibody) at dilution of 1:50 (under 40x lens)
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Background
BCMO1(Beta,beta-carotene 15,15'-monooxygenase) is also named as BCDO, BCDO1 and belongs to the carotenoid oxygenase family.The human BCMO1 cDNA encodes a 63-kDa protein with homologies to members of a large and diverse family of polyene chain oxidases and carotenoid cleavage enzymes.Although BCMO1 can be detected in several tissues, its expression is most pronounced in intestinal mucosa and liver(PMID:16504037).It catalyzes the central cleavage of β-carotene to all-trans retinal and is the key enzyme in the intestinal metabolism of carotenes to vitamin A.
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