-
Product Name
BCL2L13 antibody
- Documents
-
Description
BCL2L13 Rabbit Polyclonal antibody. Positive IF detected in HepG2 cells. Positive IHC detected in human pancreas tissue. Positive WB detected in Jurkat cells, C6 cells, HeLa cells, K-562 cells. Positive IP detected in mouse heart tissue. Observed molecular weight by Western-blot: 85kd
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat; other species not tested.
-
Alternative names
BCL2L13 antibody; MIL1 antibody; Protein Mil1 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of BCL2L13 recombinant protein (Accession Number: BC003032). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
-
Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:117098(BCL2L13 antibody) at dilution of 1:500
IP Result of anti-BCL2L13 (IP:Catalog No:117098, 3ug; Detection:Catalog No:117098 1:100) with mouse heart tissue lysate 8000ug.
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:117098(BCL2L13 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:117098(BCL2L13 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of HepG2 cells, using BCL2L13 antibody Catalog No:117098 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
C6 cells were subjected to SDS PAGE followed by western blot with Catalog No:117098(BCL2L13 Antibody) at dilution of 1:600
-
Background
BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).
-
References
- Jensen SA, Calvert AE, Volpert G. Bcl2L13 is a ceramide synthase inhibitor in glioblastoma. Proceedings of the National Academy of Sciences of the United States of America. 111(15):5682-7. 2014.
- Murakawa T, Yamaguchi O, Hashimoto A. Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation. Nature communications. 6:7527. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"