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Product Name
BCKDK Polyclonal Antibody
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Description
Polyclonal antibody to BCKDK
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
BCKDK antibody; BCKDKD antibody; BDK antibody; branched chain ketoacid dehydrogenase kinase antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 153-412 of human BCKDK (NP_005872.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:50 - 1:100 -
Validations
Western blot - BCKDK Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BCKDK antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - BCKDK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat lung using BCKDK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BCKDK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using BCKDK antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - BCKDK Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using BCKDK antibody at dilution of 1:100 (40x lens).
Immunofluorescence - BCKDK Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using BCKDK antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Catalyzes the phosphorylation and inactivation of the branched-chain alpha-ketoacid dehydrogenase complex, the key regulatory enzyme of the valine, leucine and isoleucine catabolic pathways. Key enzyme that regulate the activity state of the BCKD complex.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"