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Product Name
BCAT1 Polyclonal Antibody
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Description
Polyclonal antibody to BCAT1
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Tested applications
WB
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Species reactivity
Rat
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Alternative names
BCAT1 antibody; BCATC antibody; BCT1 antibody; ECA39 antibody; MECA39 antibody; PNAS121 antibody; PP18 antibody; branched chain amino acid transaminase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human BCAT1
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - BCAT1 Polyclonal Antibody
Western blot analysis of extracts of rat brain, using BCAT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
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Background
Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"