-
Product Name
BCAS2 antibody
- Documents
-
Description
BCAS2 Rabbit Polyclonal antibody. Positive IF detected in Hela cells, HepG2 cells, MCF-7 cells. Positive IHC detected in human colon cancer tissue, human breast cancer tissue. Positive IP detected in mouse brain tissue. Positive WB detected in MCF7 cells, COS-7 cells, HeLa cells, hESC cells, human brain tissue, K-562 cells, mouse brain tissue, mouse liver tissue, SH-SY5Y cells. Observed molecular weight by Western-blot: 26-32kd
-
Tested applications
ELISA, WB, IHC, IF, IP
-
Species reactivity
Human,Mouse,Rat, Monkey; other species not tested.
-
Alternative names
BCAS2 antibody; DAM1 antibody; Pre mRNA splicing factor SPF27 antibody
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained by immunization of BCAS2 recombinant protein (Accession Number: NM_005872). Purification method: Antigen affinity purified.
-
Clonality
Polyclonal
-
Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
-
Storage instructions
Store at -20℃. DO NOT ALIQUOT
-
Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:20-1:200
-
Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108380(BCAS2 antibody) at dilution of 1:1000
IP Result of anti-BCAS2 (IP:Catalog No:108380, 3ug; Detection:Catalog No:108380 1:1000) with mouse brain tissue lysate 6500ug.
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108380(BCAS2 antibody) at dilution of 1:100 (under 25x lens)
Immunofluorescent analysis of Hela cells, using BCAS2 antibody Catalog No:108380 at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
-
Background
BCAS2 associates with the spliceosome and implicates in mRNA splicing
-
References
- Kuo PC, Huang CW, Lee CI. BCAS2 promotes prostate cancer cells proliferation by enhancing AR mRNA transcription and protein stability. British journal of cancer. 112(2):391-402. 2015.
- Liao S, Du R, Wang L. BCAS2 interacts with HSF4 and negatively regulates its protein stability via ubiquitination. The international journal of biochemistry & cell biology. 68:78-86. 2015.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"