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Product Name
BBX antibody
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Description
BBX Rabbit Polyclonal antibody. Positive IHC detected in human ovary tissue, human brain tissue, human lung tissue, human placenta tissue, human spleen tissue. Positive WB detected in HepG2 cells. Positive IP detected in HepG2 cells. Observed molecular weight by Western-blot: 105 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BBX antibody; Bobby sox homolog antibody; bobby sox homolog (Drosophila) antibody; HBP2 antibody; HMG box containing protein 2 antibody; HSPC339 antibody; MDS001 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BBX recombinant protein (Accession Number: BC012837). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108378(BBX antibody) at dilution of 1:1500
Immunohistochemical of paraffin-embedded human ovary using Catalog No:108378(BBX antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human ovary using Catalog No:108378(BBX antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-BBX (IP:Catalog No:108378, 4ug; Detection:Catalog No:108378 1:1000) with HepG2 cells lysate 2400ug.
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Background
BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature
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References
- Chen T, Zhou L, Yuan Y. Characterization of Bbx, a member of a novel subfamily of the HMG-box superfamily together with Cic. Development genes and evolution. 224(4-6):261-8. 2014.
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