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Product Name
BBS8 antibody
- Documents
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Description
BBS8 Rabbit Polyclonal antibody. Positive IP detected in HeLa cells. Positive WB detected in mouse testis tissue, human lung tissue, NIH/3T3 cells. Observed molecular weight by Western-blot: 50-52 kDa
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Tested applications
ELISA, WB, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
BBS8 antibody; TPR repeat protein 8 antibody; TTC8 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BBS8 recombinant protein (Accession Number: NM_001288781). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:1000
IP: 1:200-1:1000
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Validations
mouse testis tissue were subjected to SDS PAGE followed by western blot with Catalog No:108376(TTC8 antibody) at dilution of 1:300
IP Result of anti-TTC8 (IP:Catalog No:108376, 3ug; Detection:Catalog No:108376 1:400) with HeLa cells lysate 2000ug.
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Background
BBS8 (also known as TTC8) is a subunit of BBSome complex which is necessary for ciliary membrane biogenesis. BBS8 localizes specifically to ciliated structures, such as the connecting cilium of the retina and columnar epithelial cells in the lung. Defects in BBS8 cause Bardet-Biedl syndrome type 8 (BBS8), an genetic disorder characterised by retinal degeneration, obesity, polydactyly, cognitive impairment and kidney failure. BBS8 generates two alternatively spliced isoforms. Western blot analysis using this antibody detected a major band around 60 kD in lung tissue.
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