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BBS10 antibody

Cat.#: 108368

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Product Information

  • Product Name
    BBS10 antibody
  • Documents
  • Description
    BBS10 Rabbit Polyclonal antibody. Positive IF detected in hTERT-RPE1 cells. Positive WB detected in HepG2 cells, A549 cells, MCF7 cells. Observed molecular weight by Western-blot: 81kd
  • Tested applications
    ELISA, WB, IF
  • Species reactivity
    Human,Zebrafish; other species not tested.
  • Alternative names
    Bardet Biedl syndrome 10 antibody; BBS10 antibody; C12orf58 antibody; FLJ23560 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of BBS10 recombinant protein (Accession Number: BC013795). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:200-1:2000

    IF: 1:20-1:200

  • Validations

    IF result (nuclear and the base of cilia stain) of anti-BBS10 (Catalog No:108368; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim.

    IF result (nuclear and the base of cilia stain) of anti-BBS10 (Catalog No:108368; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim.

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108368(BBS10 antibody) at dilution of 1:800

    HepG2 cells were subjected to SDS PAGE followed by western blot with Catalog No:108368(BBS10 antibody) at dilution of 1:800

  • Background
    BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).
  • References
    • Marion V, Stoetzel C, Schlicht D. Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation. Proceedings of the National Academy of Sciences of the United States of America. 106(6):1820-5. 2009.
    • Cognard N, Scerbo MJ, Obringer C. Comparing the Bbs10 complete knockout phenotype with a specific renal epithelial knockout one highlights the link between renal defects and systemic inactivation in mice. Cilia. 4:10. 2015.
    • Seo S, Baye LM, Schulz NP. BBS6, BBS10, and BBS12 form a complex with CCT/TRiC family chaperonins and mediate BBSome assembly. Proceedings of the National Academy of Sciences of the United States of America. 107(4):1488-93. 2010.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"