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BAX antibody

Cat.#: 107077

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Special Price 239.0 USD

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Product Information

  • Product Name
    BAX antibody
  • Documents
  • Description
    BAX Mouse Monoclonal antibody. Positive IHC detected in human breast cancer tissue, human liver cancer tissue. Positive WB detected in HEK-293 cells, human testis tissue. Observed molecular weight by Western-blot: 21 kDa
  • Tested applications
    ELISA, WB, IHC
  • Species reactivity
    Human; other species not tested.
  • Alternative names
    Apoptosis regulator BAX antibody; BAX antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    This antibody was obtained by immunization of BAX recombinant protein (Accession Number: NM_138761). Purification method: Protein A purified.
  • Clonality
    Monoclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IHC: 1:50-1:500

  • Validations

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107077(BAX antibody) at dilution of 1:1000

    HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107077(BAX antibody) at dilution of 1:1000

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107077(BAX Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107077(BAX Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107077(BAX Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

    Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using Catalog No:107077(BAX Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

  • Background
    BAX, also named as BCL2L4, is a pro-apoptotic member of the Bcl-2 protein family, which plays a pivotal role in controlling cell life and death. Bax largely localizes to the cytoplasm of healthy cells, but accumulates on the outer mitochondrial membrane upon apoptosis induction (PMID: 9108035). BAX can commit a cell to apoptosis by translocation from the cytosol to the mitochondria and permeabilization of the outer mitochondrial membrane, which leads to the release of cytochrome c from mitochondria (PMID: 21763611). The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis (PMID: 8183579).
  • References
    • Luo J, Wu N, Jiang B. Marine Bromophenol Derivative 3,4-Dibromo-5-(2-bromo-3,4-dihydroxy-6-isopropoxymethyl benzyl)benzene-1,2-diol Protects Hepatocytes from Lipid-Induced Cell Damage and Insulin Resistance via PTP1B Inhibition. Marine drugs. 13(7):4452-69. 2015.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"