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Product Name
Barkor-Specific antibody
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Description
Barkor-Specific Rabbit Polyclonal antibody. Positive IF detected in MCF-7 cells. Positive WB detected in mouse brain tissue. Positive IP detected in HeLa cells. Observed molecular weight by Western-blot: 55-60 kDa
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Tested applications
ELISA, WB, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATG14 antibody; Atg14L antibody; Barkor antibody; KIAA0831 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of Peptide (Accession Number: NM_014924). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:200-1:2000
IP: 1:200-1:2000
IF: 1:10-1:100
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Validations
mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108361(Barkor-Specific antibody) at dilution of 1:500
IP Result of anti-Barkor-Specific (IP:Catalog No:108361, 4ug; Detection:Catalog No:108361 1:500) with HeLa cells lysate 2800ug.
Immunofluorescent analysis of MCF-7 cells using Catalog No:108361(Barkor-Specific Antibody) at dilution of 1:25 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Background
Barkor, also named as KIAA0831, Atg14 and Atg14L, is required for both basal and inducible autophagy. It plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Barkor promotes BECN1 translocation from the trans-Golgi network to autophagosomes. This antibody is specific to KIAA0831(Barkor).
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References
- Fu Q, Shi H, Ren Y. Bovine viral diarrhea virus infection induces autophagy in MDBK cells. Journal of microbiology (Seoul, Korea). 52(7):619-25. 2014.
- Fu Q, Shi H, Ni W. Lentivirus-mediated Bos taurus bta-miR-29b overexpression interferes with bovine viral diarrhoea virus replication and viral infection-related autophagy by directly targeting ATG14 and ATG9A in Madin-Darby bovine kidney cells. The Journal of general virology. 96(Pt 1):85-94. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"