Product Name

Barkor antibody

Documents

Description

Barkor Rabbit Polyclonal antibody. Positive IF detected in HeLa cells. Positive IHC detected in human brain tissue, human liver tissue. Positive WB detected in mouse brain tissue. Observed molecular weight by Western-blot: 60 kDa

Tested applications

ELISA, WB, IF, IHC

Species reactivity

Human, Mouse; other species not tested.

Alternative names

ATG14 antibody; Atg14L antibody; Barkor antibody; KIAA0831 antibody

Isotype

Rabbit IgG

Preparation

This antibody was obtained by immunization of Barkor recombinant protein (Accession Number: NM_014924). Purification method: Antigen affinity purified.

Clonality

Polyclonal

Formulation

PBS with 0.1% sodium azide and 50% glycerol pH 7.3.

Storage instructions

Store at -20℃. DO NOT ALIQUOT

Applications

Recommended Dilution:

WB: 1:200-1:2000

IHC: 1:20-1:200

IF: 1:10-1:100

Validations

mouse brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108360(Barkor Antibody) at dilution of 1:600

Immunohistochemistry of paraffin-embedded human brain slide using Catalog No:108360(Barkor Antibody) at dilution of 1:50 (under 10x lens)

Immunohistochemistry of paraffin-embedded human brain slide using Catalog No:108360(Barkor Antibody) at dilution of 1:50 (under 40x lens)

Immunofluorescent analysis of HeLa cells using Catalog No:108360(Barkor Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG

Background

Barkor, also named as ATG14, Atg14L and KIAA0831, is named as Beclin 1-associated autophagy-related key regulator protein. The function of Barkor in autophagy has been manifested in several assays, including stress-induced LC3 lipidation, autophagosome formation, and Salmonella typhimurium amplification.

References

Fu Q, Shi H, Shi M. Roles of bovine viral diarrhea virus envelope glycoproteins in inducing autophagy in MDBK cells. Microbial pathogenesis. 76:61-6. 2014.

Barkor antibody

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