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Product Name
BAP31 antibody
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Description
BAP31 Rabbit Polyclonal antibody. Positive IP detected in A431 cells. Positive WB detected in HEK-293 cells, A431 cells, HeLa cells, human placenta tissue, mouse placenta tissue. Positive IF detected in Hela cells. Positive IHC detected in human colon cancer tissue. Positive FC detected in HeLa cells. Observed molecular weight by Western-blot: 28 kDa
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Tested applications
ELISA, IHC, IF, IP, FC, WB
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
6C6 AG antibody; BAP31 antibody; BCAP31 antibody; CDM antibody; DXS1357E antibody; p28 antibody; Protein CDM antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of BAP31 recombinant protein (Accession Number: NM_001256447). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:1000-1:10000
IP: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:108421(BCAP31 antibody) at dilution of 1:2000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108421(BCAP31 antibody) at dilution of 1:100 (under 10x lens)
IP Result of anti-BCAP31 (IP:Catalog No:108421, 3ug; Detection:Catalog No:108421 1:1000) with A431 cells lysate 3300ug.
1X10^6 HeLa cells were stained with 0.2ug BCAP31 antibody (Catalog No:108421, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
Immunofluorescent analysis of Hela cells, using BCAP31 antibody Catalog No:108421 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
BAP31 is a chaperone protein that is abundant in the endoplasmic reticulum (ER). BAP31 plays a role in the export of secreted proteins in the ER, the recognition of abnormally folded protein and their targeting to the ER associated-degradation (ERAD). It also serves as a cargo receptor for the export of transmembrane proteins. BAP31 may be involved in apoptosis.
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References
- Iwasawa R, Mahul-Mellier AL, Datler C, Pazarentzos E, Grimm S. Fis1 and Bap31 bridge the mitochondria-ER interface to establish a platform for apoptosis induction. The EMBO journal. 30(3):556-68. 2011.
- Lam SS, Martell JD, Kamer KJ. Directed evolution of APEX2 for electron microscopy and proximity labeling. Nature methods. 12(1):51-4. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"