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Product Name
BAG2 Polyclonal Antibody
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Description
Polyclonal antibody to BAG2
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Tested applications
WB, IHC, IF
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Species reactivity
Human, Mouse, Rat
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Alternative names
BAG2 antibody; BAG-2 antibody; dJ417I1.2 antibody; BCL2 associated athanogene 2 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-211 of human BAG2 (NP_004273.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200 -
Validations
Western blot - BAG2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using BAG2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit .Exposure time: 60s.
Immunohistochemistry - BAG2 Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human kidney using BAG2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence - BAG2 Polyclonal Antibody
Immunofluorescence analysis of U2OS cells using BAG2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
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Background
Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"