BAF250 Polyclonal Antibody

Cat.#: 163303

Special Price 174.2 USD

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Product Information

  • Product Name
    BAF250 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to BAF250
  • Tested applications
    WB
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ARID1A antibody; B120 antibody; BAF250 antibody; BAF250a antibody; BM029 antibody; C1orf4 antibody; CSS2 antibody; ELD antibody; MRD14 antibody; OSA1 antibody; P270 antibody; SMARCF1 antibody; hELD antibody; hOSA1 antibody; AT-rich interaction domain 1A antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide corresponding to a sequence within amino acids 1300-1400 of mouse BAF250 (NP_001074288.1).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
  • Validations

    Western blot - BAF250 Polyclonal Antibody

    Western blot - BAF250 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using BAF250 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 15s.

  • Background
    Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Binds DNA non-specifically. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"