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Product Name
B4GALNT2 antibody
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Description
B4GALNT2 Rabbit Polyclonal antibody. Positive WB detected in Jurkat cells, COLO 320 cells. Positive IHC detected in human colon cancer tissue, human colon tissue. Observed molecular weight by Western-blot: 65 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human; other species not tested.
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Alternative names
B4GALGT2 antibody; B4GALNT2 antibody; B4GALT antibody; Cad antibody; GALGT2 antibody; Sda antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of B4GALNT2 recombinant protein (Accession Number: NM_153446). Purification method: Antigen Affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
Jurkat cells were subjected to SDS PAGE followed by western blot with Catalog No:108392(B4GALNT2 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:108392(B4GALNT2 Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human colon cancer tissue slide using Catalog No:108392(B4GALNT2 Antibody) at dilution of 1:50 (under 40x lens)
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Background
B4GALNT2, also named as GALGT2, catalyzes the last step in the biosynthesis of the human Sd(a) antigen, which is found on more than 90% of Caucasian red blood cells. It also catalyzes the last step in the biosynthesis of the Cad antigen, which is related both serologically and biochemically to the Sd(a) antigen. B4GALNT2 transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. This antibody detects the 55-65 kDa B4GALNT2 protein.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"