Human BLNK/Ly-57/SLP-65 (His Tag) recombinant protein

Human BLNK/Ly-57/SLP-65 (His Tag) recombinant protein

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Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

B-cell linker protein

SLP65; BLNK-S; BASH; LY57; MGC111051; AGM4; BCA; LY-57; BLNK; SLP-65; LYW-57

Q8WV28

BLNK; BASH; SLP65

Human Cells

A DNA sequence encoding the human BLNK isoform 1 (AAH18906.1) (Met 1-Ser 456) was fused with a polyhistidine tag at the N-terminus.

Human

The recombinant human BLNK consists of 476 amino acids and has a calculated molecular mass of 53 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh BLNK is approximately 95-100 kDa due to glycosylation.

> 90 % as determined by SDS-PAGE

Measured by its ability to bind human BTK in a functional ELISA.