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Product Name
AUP1 antibody
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Description
AUP1 Rabbit Polyclonal antibody. Positive WB detected in K-562 cells, HEK-293 cells, Jurkat cells. Positive IP detected in HEK-293 cells. Positive IHC detected in human liver cancer tissue. Positive IF detected in A431 cells. Observed molecular weight by Western-blot: 40kd
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Tested applications
ELISA, WB, IHC, IF, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ancient ubiquitous protein 1 antibody; AUP1 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of AUP1 recombinant protein (Accession Number: BC033646). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
K-562 cells were subjected to SDS PAGE followed by western blot with Catalog No:117072(AUP1 antibody) at dilution of 1:400
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:117072(AUP1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver cancer using Catalog No:117072(AUP1 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-AUP1 (IP:Catalog No:117072, 3ug; Detection:Catalog No:117072 1:500) with HEK-293 cells lysate 880ug.
Immunofluorescent analysis of A431 cells using Catalog No:117072(AUP1 Antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG
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Background
AUP1 represents ancient ubiquitous protein 1
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References
- Saka HA, Thompson JW, Chen YS. Chlamydia trachomatis Infection Leads to Defined Alterations to the Lipid Droplet Proteome in Epithelial Cells. PloS one. 10(4):e0124630. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"