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Product Name
ATP7A Polyclonal Antibody
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Description
Polyclonal antibody to ATP7A
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Tested applications
WB, IHC
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Species reactivity
Human, Rat
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Alternative names
ATP7A antibody; DSMAX antibody; MK antibody; MNK antibody; SMAX3 antibody; copper-transporting ATPase 1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human ATP7A (NP_000043.4).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:100 -
Validations
Western blot - ATP7A Polyclonal Antibody
Western blot analysis of extracts of 293T cells, using ATP7A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunohistochemistry - ATP7A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded rat kidney using ATP7A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ATP7A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human liver injury using ATP7A antibody at dilution of 1:100 (40x lens).
Immunohistochemistry - ATP7A Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using ATP7A antibody at dilution of 1:100 (40x lens).
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Background
May supply copper to copper-requiring proteins within the secretory pathway, when localized in the trans-Golgi network. Under conditions of elevated extracellular copper, it relocalized to the plasma membrane where it functions in the efflux of copper from cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"