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Product Name
ATP6V1F antibody
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Description
ATP6V1F Rabbit Polyclonal antibody. Positive FC detected in MCF-7 cells. Positive IHC detected in human testis tissue, human brain tissue, human kidney tissue, human pancreas tissue, human placenta tissue. Positive WB detected in MCF7 cells, HeLa cells, Jurkat cells, mouse liver tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 14 kDa
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Tested applications
ELISA, WB, IHC, FC
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Species reactivity
Human, Mouse; other species not tested.
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Alternative names
ATP6S14 antibody; ATP6V1F antibody; V ATPase 14 kDa subunit antibody; V ATPase subunit F antibody; V type proton ATPase subunit F antibody; Vacuolar proton pump subunit F antibody; VATF antibody; Vma7 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP6V1F recombinant protein (Accession Number: NM_004231). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
MCF7 cells were subjected to SDS PAGE followed by western blot with Catalog No:108313(ATP6V1F antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human testis using Catalog No:108313(ATP6V1F antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human testis using Catalog No:108313(ATP6V1F antibody) at dilution of 1:50 (under 40x lens)
1X10^6 MCF-7 cells were stained with 0.2ug ATP6V1F antibody (Catalog No:108313, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
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Background
ATP6V1F(V-type proton ATPase subunit F) is also named as ATP6S14, VATF and belongs to the V-ATPase F subunit family. It generates an electrochemical proton gradient that is acid and positive inside synaptic vesicles. ATP6V1F plays a major role as energizers of animal plasma membranes, especially apical plasma membranes of epithelial cells. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 14 kDa and 16 kDa.
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