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  • ATP6V1E1 antibody
  • ATP6V1E1 antibody
  • ATP6V1E1 antibody
  • ATP6V1E1 antibody

ATP6V1E1 antibody

Cat.#: 108311

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Product Information

  • Product Name
    ATP6V1E1 antibody
  • Documents
  • Description
    ATP6V1E1 Rabbit Polyclonal antibody. Positive IHC detected in human testis tissue. Positive WB detected in human brain tissue, HEK-293 cells, HeLa cells, human liver tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 31kd
  • Tested applications
    ELISA, WB, IHC, IP
  • Species reactivity
    Human,Mouse,Rat; other species not tested.
  • Alternative names
    ATP6E antibody; ATP6E2 antibody; ATP6V1E antibody; ATP6V1E1 antibody; P31 antibody; V ATPase 31 kDa subunit antibody; V ATPase subunit E 1 antibody; Vma4 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antibody was obtained by immunization of ATP6V1E1 recombinant protein (Accession Number: BC004443). Purification method: Antigen affinity purified.
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
  • Storage instructions
    Store at -20℃. DO NOT ALIQUOT
  • Applications

    Recommended Dilution:

    WB: 1:500-1:5000

    IP: 1:200-1:2000

    IHC: 1:20-1:200

  • Validations

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:500

    human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:500

    Immunohistochemical of paraffin-embedded human testis using Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:100 (under 40x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:100 (under 10x lens)

    Immunohistochemical of paraffin-embedded human testis using Catalog No:108311(ATP6V1E1 antibody) at dilution of 1:100 (under 10x lens)

    IP Result of anti-ATP6V1E1 (IP:Catalog No:108311, 3ug; Detection:Catalog No:108311 1:500) with mouse brain tissue lysate 6000ug.

    IP Result of anti-ATP6V1E1 (IP:Catalog No:108311, 3ug; Detection:Catalog No:108311 1:500) with mouse brain tissue lysate 6000ug.

  • Background
    ATP6V1E1, also named as ATP6E, ATP6E2 and p31, belongs to the V-ATPase E subunit family. It is a subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. V-ATPase and the lack of mycobacterial phagosome acidification are directly attributed to the Mtb protein tyrosine phosphatase PtpA.
  • References
    • Wong D, Bach H, Sun J, Hmama Z, Av-Gay Y. Mycobacterium tuberculosis protein tyrosine phosphatase (PtpA) excludes host vacuolar-H+-ATPase to inhibit phagosome acidification. Proceedings of the National Academy of Sciences of the United States of America. 108(48):19371-6. 2011.
    • Yang LS, Xu XE, Liu XP. iTRAQ-based quantitative proteomic analysis for identification of oligodendroglioma biomarkers related with loss of heterozygosity on chromosomal arm 1p. Journal of proteomics. 77:480-91. 2012.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"