ATP6V1B2 Polyclonal Antibody
Polyclonal antibody to ATP6V1B2
Human, Mouse, Rat
ATP6V1B2 antibody; ATP6B1B2 antibody; ATP6B2 antibody; DOOD antibody; HO57 antibody; VATB antibody; VPP3 antibody; Vma2 antibody; ZLS2 antibody; ATPase H+ transporting V1 subunit B2 antibody
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2).
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Store at -20℃. Avoid freeze / thaw cycles.
WB 1:500 - 1:2000
IF 1:50 - 1:200
Western blot - ATP6V1B2 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATP6V1B2 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunofluorescence - ATP6V1B2 Polyclonal Antibody
Immunofluorescence analysis of HeLa cells using ATP6V1B2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"