ATP6V1B2 Polyclonal Antibody

Cat.#: 164196

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Product Information

  • Product Name
    ATP6V1B2 Polyclonal Antibody
  • Documents
  • Description
    Polyclonal antibody to ATP6V1B2
  • Tested applications
    WB, IF
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    ATP6V1B2 antibody; ATP6B1B2 antibody; ATP6B2 antibody; DOOD antibody; HO57 antibody; VATB antibody; VPP3 antibody; Vma2 antibody; ZLS2 antibody; ATPase H+ transporting V1 subunit B2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 262-511 of human ATP6V1B2 (NP_001684.2).
  • Clonality
    Polyclonal
  • Formulation
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
  • Storage instructions
    Store at -20℃. Avoid freeze / thaw cycles.
  • Applications
    WB 1:500 - 1:2000
    IF 1:50 - 1:200
  • Validations

    Western blot - ATP6V1B2 Polyclonal Antibody

    Western blot - ATP6V1B2 Polyclonal Antibody

    Western blot analysis of extracts of various cell lines, using ATP6V1B2 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

    Immunofluorescence - ATP6V1B2 Polyclonal Antibody

    Immunofluorescence - ATP6V1B2 Polyclonal Antibody

    Immunofluorescence analysis of HeLa cells using ATP6V1B2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

  • Background
    Non-catalytic subunit of the peripheral V1 complex of vacuolar ATPase. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"