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Product Name
ATP6V1B2 antibody
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Description
ATP6V1B2 Rabbit Polyclonal antibody. Positive IHC detected in human colon cancer tissue. Positive WB detected in HeLa cells, mouse brain tissue, mouse heart tissue, mouse skeletal muscle tissue. Positive IP detected in mouse brain tissue. Observed molecular weight by Western-blot: 56 kDa, 58 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP6B1B2 antibody; ATP6B2 antibody; ATP6V1B2 antibody; HO57 antibody; V ATPase subunit B 2 antibody; VATB antibody; Vma2 antibody; VPP3 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP6V1B2 recombinant protein (Accession Number: NM_001693). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:500-1:5000
IHC: 1:20-1:200
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Validations
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:108307(ATP6V1B2 antibody) at dilution of 1:3000
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108307(ATP6V1B2 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human colon cancer using Catalog No:108307(ATP6V1B2 antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ATP6V1B2 (IP:Catalog No:108307, 4ug; Detection:Catalog No:108307 1:1000) with mouse brain tissue lysate 4000ug.
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Background
ATP6V1B2(V-type proton ATPase subunit B, brain isoform) is also named as ATP6B2, VPP3,HO57. It belongs to the ATPase alpha/beta chains family.V-ATPase is an heteromultimeric enzyme composed of a peripheral catalytic V1 complex attached to an integral membrane V0 proton pore complex.The quasi-ubiquitous ATP6V1B2 is one of 2 isoforms of ATP6V1 and is expressed in most cell types, where it plays a major role in organelle acidification(PMID:18667600).
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