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Product Name
ATP6V1A antibody
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Description
ATP6V1A Rabbit Polyclonal antibody. Positive WB detected in mouse kidney tissue, HeLa cells, human placenta tissue, K-562 cells. Positive IP detected in HeLa cells. Positive IHC detected in human pancreas tissue. Observed molecular weight by Western-blot: 68 kDa
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Tested applications
ELISA, WB, IHC, IP
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP6A1 antibody; ATP6V1A antibody; ATP6V1A1 antibody; HO68 antibody; V ATPase 69 kDa subunit antibody; V ATPase subunit A antibody; VA68 antibody; Vacuolar ATPase isoform VA68 antibody; Vma1 antibody; VPP2 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP6V1A recombinant protein (Accession Number: XM_047448306). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IP: 1:200-1:2000
IHC: 1:20-1:200
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Validations
mouse kidney tissue were subjected to SDS PAGE followed by western blot with Catalog No:108305(ATP6V1A antibody) at dilution of 1:500
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:108305(ATP6V1A antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human pancreas using Catalog No:108305(ATP6V1A antibody) at dilution of 1:100 (under 40x lens)
IP Result of anti-ATP6V1A (IP:Catalog No:108305, 4ug; Detection:Catalog No:108305 1:500) with HeLa cells lysate 4000ug.
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Background
The vacuolar-type H(+)-ATPase (V-ATPase) is responsible for the acidification of endosomes, lysosomes, and other intracellular organelles. It is also involved in hydrogen ion transport across the plasma membrane into the extracellular space. The V-ATPase is a multisubunit complex with cytosolic and transmembrane domains. The cytosolic catalytic domain consists of 3 A subunits and 3 B subunits, which bind and hydrolyze ATP, as well as regulatory accessory subunits.ATP6V1A is V-type proton ATPase catalytic subunit A.
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References
- Einhorn Z, Trapani JG, Liu Q, Nicolson T. Rabconnectin3α promotes stable activity of the H+ pump on synaptic vesicles in hair cells. The Journal of neuroscience : the official journal of the Society for Neuroscience. 32(32):11144-56. 2012.
- Gokey JJ, Dasgupta A, Amack JD. The V-ATPase accessory protein Atp6ap1b mediates dorsal forerunner cell proliferation and left-right asymmetry in zebrafish. Developmental biology. 407(1):115-30. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"