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Product Name
ATP6V0A4 Polyclonal Antibody
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Description
Polyclonal antibody to ATP6V0A4
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Tested applications
WB
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Species reactivity
Human, Mouse
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Alternative names
ATP6V0A4 antibody; A4 antibody; ATP6N1B antibody; ATP6N2 antibody; RDRTA2 antibody; RTA1C antibody; RTADR antibody; STV1 antibody; VPH1 antibody; VPP2 antibody; ATPase H+ transporting V0 subunit a4 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human ATP6V0A4 (NP_065683.2).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
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Validations
Western blot - ATP6V0A4 Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATP6V0A4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
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Background
Part of the proton channel of the V-ATPase that is involved in normal vectorial acid transport into the urine by the kidney.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"