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Product Name
ATP5I antibody
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Description
ATP5I Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue. Positive WB detected in human liver tissue, HeLa cells, mouse liver tissue, mouse ovary tissue, rat ovary tissue. Observed molecular weight by Western-blot: 8 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP5I antibody; ATP5K antibody; ATPase subunit e antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP5I recombinant protein (Accession Number: NM_007100). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
human liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108356(ATP5I antibody) at dilution of 1:300
Immunohistochemical of paraffin-embedded human liver using Catalog No:108356(ATP5I antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:108356(ATP5I antibody) at dilution of 1:50 (under 40x lens)
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Background
ATP5I(ATP synthase subunit e) is also named as ATP5K and belongs to the ATPase e subunit family. The ATP5I gene encodes the e subunit of the mitochondrial ATP synthase Fo complex. Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. Antisense ATP5I in a human HCC cell line inhibited cell growth suggesting that ATP5I acts through the MAP kinase pathway(PMID:11939412).
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