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Product Name
ATP5F1 antibody
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Description
ATP5F1 Rabbit Polyclonal antibody. Positive IHC detected in human liver tissue, human brain tissue, human heart tissue, human kidney tissue, human skin tissue, human testis tissue. Positive IF detected in Hela cells. Positive WB detected in mouse liver tissue, mouse heart tissue, mouse skeletal muscle tissue. Observed molecular weight by Western-blot: 25kd
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Tested applications
ELISA, WB, IHC, IF
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP5F1 antibody; ATPase subunit b antibody; PIG47 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP5F1 recombinant protein (Accession Number: NM_001688). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
IF: 1:10-1:100
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Validations
mouse liver tissue were subjected to SDS PAGE followed by western blot with Catalog No:108353(ATP5F1 antibody) at dilution of 1:1000
Immunohistochemical of paraffin-embedded human liver using Catalog No:108353(ATP5F1 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human liver using Catalog No:108353(ATP5F1 antibody) at dilution of 1:100 (under 40x lens)
Immunofluorescent analysis of Hela cells, using ATP5F1 antibody Catalog No:108353 at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
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Background
ATP5F1(ATP synthase subunit b) belongs to the eukaryotic ATPase B chain family. The ATP5F1 gene encodes subunit B of the mitochondrial ATP synthase Fo unit, which contains 214-amino acid with a 42-amino acid import signal(PMID:1831354). Mitochondrial membrane ATP synthase(F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain.
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References
- Zhang J, Sun M, Li R. Ech1 is a potent suppressor of lymphatic metastasis in hepatocarcinoma. Biomedicine & pharmacotherapy = Biomédecine & pharmacothérapie. 67(7):557-60. 2013.
- Kioka H, Kato H, Fujikawa M. Evaluation of intramitochondrial ATP levels identifies G0/G1 switch gene 2 as a positive regulator of oxidative phosphorylation. Proceedings of the National Academy of Sciences of the United States of America. 111(1):273-8. 2014.
- Liu C, Lin JJ, Yang ZY, Tsai CC, Hsu JL, Wu YJ. Proteomic study reveals a co-occurrence of gallic acid-induced apoptosis and glycolysis in B16F10 melanoma cells. Journal of agricultural and food chemistry. 62(48):11672-80. 2014.
- Comelli M, Domenis R, Buso A, Mavelli I. F1 FO ATP Synthase Is Expressed at the Surface of Embryonic Rat Heart-Derived H9c2 Cells and Is Affected by Cardiac-Like Differentiation. Journal of cellular biochemistry. 117(2):470-82. 2016.
- Fujikawa M, Sugawara K, Tanabe T, Yoshida M. Assembly of human mitochondrial ATP synthase through two separate intermediates, F1-c-ring and b-e-g complex. FEBS letters. 589(19 Pt B):2707-12. 2015.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"