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Product Name
ATP5D antibody
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Description
ATP5D Rabbit Polyclonal antibody. Positive WB detected in A549 cells, NIH/3T3 cells. Positive IHC detected in human kidney tissue, human lung cancer tissue. Observed molecular weight by Western-blot: 15-17 kDa
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Tested applications
ELISA, WB, IHC
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Species reactivity
Human,Mouse,Rat; other species not tested.
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Alternative names
ATP5D antibody; F ATPase delta subunit antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained by immunization of ATP5D recombinant protein (Accession Number: NM_001001975). Purification method: Antigen affinity purified.
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IHC: 1:20-1:200
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Validations
A549 cells were subjected to SDS PAGE followed by western blot with Catalog No:108352(ATP5D antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:108352(ATP5D Antibody) at dilution of 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human kidney tissue slide using Catalog No:108352(ATP5D Antibody) at dilution of 1:50 (under 40x lens)
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Background
The mitochondrial F1Fo-ATP synthase complex uses energy derived from a proton gradient to synthesize ATP. The ATP5D gene encodes the delta subunit of the mitochondrial ATP synthase F1 complex and belongs to the ATPase epsilon chain family. This gene encode the full length protein of 17 kDa with a transit peptide of 22 amino acid.
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