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Product Name
ATP4B Polyclonal Antibody
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Description
Polyclonal antibody to ATP4B
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Tested applications
WB, IHC
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Species reactivity
Human, Mouse, Rat
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Alternative names
ATP4B antibody; ATP6B antibody; ATPase H+/K+ transporting beta subunit antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant fusion protein containing a sequence corresponding to amino acids 62-291 of human ATP4B (NP_000696.1).
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Clonality
Polyclonal
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Formulation
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
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Storage instructions
Store at -20℃. Avoid freeze / thaw cycles.
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Applications
WB 1:500 - 1:2000
IHC 1:50 - 1:200 -
Validations
Western blot - ATP4B Polyclonal Antibody
Western blot analysis of extracts of various cell lines, using ATP4B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.
Immunohistochemistry - ATP4B Polyclonal Antibody
Immunohistochemistry of paraffin-embedded human stomach using ATP4B antibody at dilution of 1:100 (40x lens).
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Background
Required for stabilization and maturation of the catalytic proton pump alpha subunit and may also involved in cell adhesion and establishing epithelial cell polarity.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"